biology the role of ceacam in LOVO AND LS174T CELLS

Project description

1. introduction (about 500 words

introduce the colorectal CANCER cell lines : HCT116/ LOVO/LS174T
introduce BCL-3.
introduce CEACAM (especially ceacam 1/ ceacam 6/ CEA)

2. meterial and method about 1000

3. results (about 2000-2500
a.what is the best MOI to infect the HCT116 cells(MOI=50-100)
b.what is the best time point for HCT116 cells (Not well regulated, 24h seems better)
c. what is the role of ceacam? (4 subspecies at 24h , find out 24h maybe not the best time point for LOVO AND LS174T )
d. what is the best time point for LOVO AND LS174T cells (WELL REGULATED. 8H is the best.)
e.what is the role of ceacam in LOVO AND LS174T CELLS? (4 SUBSPECIES at 8h)

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